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Ngwa njirimara nwoke na nwanyị

Mpempe njirimara nwoke na nwanyị egosipụtara onyonyo

Nkọwa dị mkpirikpi:

Usoro mmeghachi omume nke kit a nwere ụzọ ụzọ nduru kpọmkwem, DNA nduru na-abawanye site na usoro PCR nkịtị, ngwaahịa ndị a na-amụbawanye na-edobe agarose gel electrophoresis. Ihe oyiyi fifinal electrophoresis nwere ike ikpebi nwoke na nwanyị nke nduru.

KPỌTỤRỤ ANYỊ

Nkọwa ngwaahịa

Mkpado ngwaahịa

Atụmatụ

1,Ngwakọta reagent dị mfe ma dị mfe iji rụọ ọrụ

2,Oke izi ezi

3,Nchekwa na nke anaghị egbu egbu, na-enweghị reagents na-egbu egbu

4,Ọ dịghị ihe ọjọọ na kpalakwukwu

Nkọwapụta ngwaahịa

Aha ngwaahịa

Nwamba. Mba

Nkọwapụta

Nkọwa

Okwu

Ngwa njirimara nwoke na nwanyị

BFRD005

50Nnwale/igbe

Ọ dị mfe iji rụọ ọrụ, ọdabara na BIGFISHQuantFinder48/96 Ngwa PCR dị adị

Maka Nnyocha

Jiri naanị

Nsonaazụ nnwale

Ngwakọta mmụba DNA doro anya, enweghị

distortion ma ọ bụ doro anya trailing.The mmekọahụ nke nnụnụ

enwere ike mata nke ọma.

Nke gara aga: FastCycler Thermal Cycler FC-96GE

Osote: Nọmba nlereanya: BFQP-48

Dee ozi gị ebe a ziga anyị ya

Ụdị ngwaahịa