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Ngwa ihe nhicha DNA nke anụ mmiri MagaPure
Okwu mmeghe dị nkenke
Ngwa a na-anabata usoro nchekwa pụrụ iche nke mepere emepe na nke kachasị mma yana bead magnetik na-ejikọ kpọmkwem na DNA. Ọ nwere ike jikọta ngwa ngwa, gbasaa, kewapụ, na sachaa acids nucleic, ma ahaziri ya maka anụmanụ ndị dị n'ime mmiri. Ọ dabara nke ọma maka ịwepụta na sachapụ DNA genomic nke ọma site na anụ ahụ anụ mmiri dị iche iche, ma nwee ike wepu adịghị ọcha dịka protein, abụba na ogige ndị ọzọ dị ndụ ruo oke o kwere mee. Site n'ịkwado iji Bigfish Magnetic Bead Nucleic Acid Extractor, ọ dabara nke ọma maka mmịpụta akpaaka nke nnukwu nlele. DNA genomic amịpụtara nwere ịdị ọcha na ịdị mma dị mma, enwere ike iji ya mee ihe na PCR / qPCR, NGS, ngwakọ ndịda na nyocha nnwale ndị ọzọ.
Atụmatụ ngwaahịa
◆ Nlere anya nke ọma: Enwere ike ị nweta DNA genomic ozugbo site na ụdị anụmanụ dị iche iche nke mmiri.
◆ Nchekwa na nke na-adịghị egbu egbu: Reagent enweghị ihe mgbaze na-egbu egbu dị ka phenol na chloroform, yana ihe nchekwa dị elu.
◆ Automation: Ejiri ya na Bigfish Nucleic Acid Extractor, ọ nwere ike ịrụpụta mmịpụta dị elu, ọkachasị dabara adaba maka iwepụta oke nha.
◆ Ịdị ọcha dị elu: enwere ike iji ya mee ihe maka nyocha ihe omimi ihe omimi dị ka PCR, mgbaze enzyme, ngwakọ, wdg.
Usoro maka mmịpụta
Nlele: Were 25-30 mg anụ ahụ anụ mmiri
Egweri: mmiri mmiri nitrogen egweri, grinder egweri ma ọ bụ ịkwa osisi
mgbaze: 56 ℃ ọkụ bat mgbaze
Na igwe: Centrifuge supernatant ma tinye ya na efere maka mmịpụta
Usoro nka
Ihe atụ nke sage: 25-30mg
DNA dị ọcha: A260/280≧1.75
Ngwa nwere ike imeghari
Bigfish BFEX-32/BFEX-32E/BFEX-96E
Nkọwapụta ngwaahịa
| Aha ngwaahịa | Nwamba. Mba. | Nkwakọ ngwaahịa |
| MagaDị ọchaGenomic anụmanụ mmiriNgwa DNA dị ọcha(pngwugwu ọzọ juru) | BFMP21R | 32T |
| MagaDị ọchaGenomic anụmanụ mmiriNgwa DNA dị ọcha (ngwungwu ejuruburu) | BFMP21R1 | 40T |
| MagaDị ọchaGenomic anụmanụ mmiriNgwa DNA dị ọcha (ngwungwu ejuruburu) | BFMP21R96 | 96T |
| RNase A(pichupu) | BFRD017 | 1 ml/tube (10 mg / ml) |
Ntọala nzuzo