Ngwa Nchacha DNA nke MagaPure Oryza sativa L.

Nkọwa Dị Mkpirikpi:

Ngwa a na-eji sistemụ nchekwa pụrụ iche emepụtara ma hazie ya na mkpụrụ osisi magnetik nke na-ejikọ kpọmkwem na DNA, nke nwere ike ijikọ ngwa ngwa, na-amịkọrọ, kewapụ ma na-asachapụ asịd nucleic, ebe ọ na-ewepụ ihe ndị na-adịghị ọcha dị ka polysaccharides na polyphenol complexes n'ime osisi nke ọma. Ọ dị mma nke ukwuu maka iwepụ DNA genomic site na anụ ahụ akwụkwọ osisi. Site n'ịkwado ojiji nke Bigfish Magnetic Bead Nucleic Acid Extractor, ọ dabara nke ọma maka mwepụta akpaaka nke nnukwu nha nlele. DNA genomic ewepụtara nwere ịdị ọcha dị elu na ezigbo mma, enwere ike iji ya mee ihe nke ukwuu na PCR/qPCR, NGS na nyocha nnwale ndị ọzọ.

KPỌTỤRỤ ANYỊ

Nkọwa Ngwaahịa

Akara Ngwaahịa

Okwu mmeghe dị mkpirikpi

Atụmatụ nke ngwaahịa

◆ Nchekwa na anaghị egbu egbu: Achọghị ihe ndị na-egbu egbu dịka phenol/chloroform
◆ Mmepụta dị elu na akpaghị aka: E ji Bigfish Nucleic Acid Extractor mee ya, ọ nwere ike ime mwepụ dị elu ma dabara adaba maka iwepụta nnukwu nha ihe nlele
◆ Ịdị ọcha dị elu na ịdị mma dị mma: Ngwaahịa a ewepụtara nwere ịdị ọcha dị elu ma enwere ike iji ya mee ihe maka NGS dị n'okpuru ala, njikọta chip na nnwale ndị ọzọ.

Usoro maka mwepụ

Nnwale: Ihe nlele ọhụrụ dị ihe dị ka 100 mg ma ọ bụ ihe nlele arọ akọrọ dị ihe dị ka 30 mg
Ịgweri: Ịgweri ya nke ọma site na iji mmiri nitrogen ma ọ bụ ihe e ji egweri ihe
Mgbaze: 65℃ mmiri ọkụ ịsa ahụ
N'ime igwe ahụ: Tinye ihe na-asọfe n'ime centrifuge ma tinye ya na efere ahụ maka mwepụta ya

Ngwa ọrụ enwere ike ịgbanwe agbanwe

Nnukwu azụ BFEX-32/BFEX-32E/BFEX-96E

Nkọwapụta nke ngwaahịa

Aha Ngwaahịa	Pusi. Mba.	Nkwakọ ngwaahịa
MagaDị ọchaOryza sativa L.Ngwa Nchacha DNA nke Genomic(pngwugwu e mejugharịrị)	BFMP23R	32T
MagaDị ọchaOryza sativa L.Ngwa Nchacha DNA nke Genomic (ngwugwu e tinyere tupu oge eruo)	BFMP23R96	96T
Proteinase K (purchase)	BFRD007	1ml/tube (10mg/ml)
RNase A(purchase)	BFRD017	1ml/tube (10mg/ml)