Ngwa dị ọcha nke MagPure Plasmid DNA

Ngwa ihe nhicha DNA MagPure Plasmid egosipụtara

Nkọwa dị mkpirikpi:

Ngwa a na-anabata usoro nchekwa pụrụ iche nke mepere emepe na nke kachasị mma yana beads magnetik nke na-ejikọ kpọmkwem na DNA, nke nwere ike jikọta ngwa ngwa, gbasaa, kewapụ ma sachaa acid nucleic. Ọ dabara nke ọma ngwa ngwa na nke ọma ikewapụ na sachapụ DNA plasmid site na 0.5-2mL (nke a na-ahụkarị 1-1.5mL) mmiri mmiri nje, ebe ọ na-ewepụ ihe ndị dị ka protein na ion nnu. Site n'ịkwado iji Bigfish Magnetic Bead Nucleic Acid Extractor, ọ dabara nke ọma maka mmịpụta akpaaka nke nnukwu nlele. DNA plasmid amịpụtara nwere ịdị ọcha dị mma na ịdị mma, enwere ike iji ya mee ihe na nnwale dị n'okpuru dị ka mgbaze enzyme, ligation, mgbanwe, NGS, wdg..

KPỌTỤRỤ ANYỊ

Nkọwa ngwaahịa

Mkpado ngwaahịa

Okwu mmeghe dị nkenke

Njirimara ngwaahịa

Ogo dị mma:Kewapụ ma sachaa DNA plasmid site na ngwọta nje bacteria 0.5-2mL nwere nnukwu mkpụrụ na ịdị ọcha dị mma..

Ngwa na mfe:Usoro niile anaghị achọ centrifugation ugboro ugboro ma ọ bụ ọrụ filtration, na-eme ka ọ dị mma maka iwepụta nnukwu nha nha..

Nchekwa na anaghị egbu egbu:Enweghị mkpa maka reagents organic na-egbu egbu dị ka phenol/chloroform.

Enwere ike imeghariNgwa ngwa

Nnukwu azụ: BFEX-32E, BFEX-32, BFEX-96E, BFEX-16E

Nkọwapụta ngwaahịa

NgwaahịaNeme	Nwamba. Mba.	Nkwakọ ngwaahịa
Ngwa dị ọcha MagPure Plasmid DNA (ngwungwu ejururịrị)	BFMP09R	32T
Ngwa dị ọcha MagPure Plasmid DNA (ngwungwu ejururịrị)	BFMP09R1	40T
Ngwa dị ọcha MagPure Plasmid DNA (ngwungwu ejururịrị)	BFMP09R96	96T
RNaseA(zuo)	BFRD017	1 ml / tube(10 mg / ml)