Ngwa MagPure ngụkọta RNA dị ọcha

Akụrụngwa MagPure ngụkọta RNA egosipụtara onyonyo

Nkọwa dị mkpirikpi:

Ngwa a na-anabata usoro nchekwa pụrụ iche nke mepere emepe na nke kachasị mma yana beads magnetik nke na-ejikọ kpọmkwem na DNA, nke nwere ike jikọta ngwa ngwa, gbasaa, kewapụ ma sachaa acid nucleic. Ọ dabara nke ọma maka ikewapụ na sachapụ RNA site na anụ ahụ na sel. Site n'ịkwado iji Bigfish Magnetic Bead Nucleic Acid Extractor, ọ dabara nke ọma maka mmịpụta akpaaka nke nnukwu nlele. Ngwaahịa nucleic acid ewepụtara nwere ịdị ọcha dị elu yana ezigbo mma, enwere ike iji ya mee ihe na mgbada RT-PCR/RT qPCR, NGS na nyocha nnwale ndị ọzọ.

KPỌTỤRỤ ANYỊ

Nkọwa ngwaahịa

Mkpado ngwaahịa

Atụmatụ

Nlereanya sara mbara:enwere ike itinye ya n'ọtụtụ ebe na anụ ahụ anụmanụ dị iche iche na mkpụrụ ndụ omenala

Nchekwa na anaghị egbu egbu:Enweghị mkpa maka reagents na-egbu egbu dị ka phenol/chloroform, nchekwa na ntụkwasị obi

Ntinye dị elu akpaaka:Ejikọtara ya na Beagle sequencing nucleic acid extractor, ọ nwere ike ịrụpụta mmịpụta dị elu ma dabara adaba maka iwepụta nnukwu nha nlele.

Ịdị ọcha dị elu na ezigbo mma:Ngwaahịa a amịpụtara nwere ịdị ọcha dị elu yana enwere ike iji ya mee nnwale mgbada dị ka RT PCR/RT qPCR na NGS.

Enwere ike imeghari Ngwa ngwa

Nnukwu azụ: BFEX-32E, BFEX-32, BFEX-16E,BFEX-96E

Nkọwapụta ngwaahịa

NgwaahịaNeme	Nwamba. Mba.	Nkwakọ ngwaahịa
MagaPureTotal RNAPume ọhụrụKya (ngwugwu juru n'ihu)	BFMP07R	32T
MagaPureTotal RNAPume ọhụrụKya (ngwugwu juru n'ihu)	BFMP07R1	40T
MagaPureTotal RNAPume ọhụrụKya (ngwugwu juru n'ihu)	BFMP07R96	96T
DNAse I (zuo)	BFRD009	1 ml/tube(5U/ul)