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Osikapa bụ otu n'ime ihe ọkụkụ ndị kacha mkpa, nke sitere na osisi ahịhịa mmiri nke ezinụlọ Poaceae. China bụ otu n'ime ebe obibi osikapa mbụ, nke a na-akọkarị na ndịda China na mpaghara ugwu ọwụwa anyanwụ. Site na ọganihu nke sayensị na teknụzụ, a na-eji usoro bayoloji nke oge a eme ihe nke ọma na nyocha osikapa. Inweta DNA genomic osikapa dị elu, nke dị ọcha nke ukwuu na-etinye ntọala siri ike maka ọmụmụ mkpụrụ ndụ ihe nketa dị n'okpuru ala. Ngwa nhicha DNA nke BigFish Sequence Magnetic Bead-Based Rice Genomic DNA na-enyere ndị nchọpụta osikapa aka iwepụta DNA osikapa ngwa ngwa, ngwa ngwa, na nke ọma.
Ngwa Nhicha DNA nke Rice Genome
Nchịkọta Ngwaahịa:
Ngwaahịa a na-eji sistemụ nchekwa pụrụ iche emepụtara ma hazie ya nke ọma yana ihe ndọta magnetik nwere njirimara njikọ DNA pụrụ iche. Ọ na-ejikọ, na-amịkọrọ, ma na-ekewapụ nucleic acid ngwa ngwa ma na-ewepụ ihe ndị na-adịghị ọcha dị ka polysaccharides na polyphenolic compounds n'ime osisi nke ọma. Ọ dị mma nke ukwuu maka iwepụ genomic DNA site na anụ ahụ akwụkwọ osisi. Ejikọtara ya na BigFish Magnetic Bead Nucleic Acid Extraction Instrument, ọ dị mma maka iwepụ nnukwu mpịakọta ihe nlele akpaka. Ngwaahịa nucleic acid ewepụtara na-egosipụta ịdị ọcha dị elu na ịdị mma dị mma, na-eme ka ha dabara adaba maka nyocha nnwale dị n'okpuru ala dịka PCR/qPCR na NGS.
Atụmatụ Ngwaahịa:
Nchekwa na anaghị egbu egbu: Achọghị ihe ndị na-egbu egbu dịka phenol/chloroform
Mmepụta dị elu na akpaghị aka: Ejikọtara ya na ihe na-ewepụta ihe na-eme ka ihe na-emepụta ihe na-eme ka ihe dị n'ime Beagle dị mfe, ọ nwere ike ime mwepụta dị elu ma dabara adaba maka iwepụta nnukwu nha ihe nlele.
Ịdị ọcha dị elu na ịdị mma dị mma: Ngwaahịa a ewepụtara nwere ịdị ọcha dị elu ma enwere ike iji ya mee ihe maka NGS dị ala, njikọta chip na nnwale ndị ọzọ.
Ngwa egwu dakọtara: BigFish BFEX-32/BFEX-32E/BFEX-96E
Oge ozi: Sep-11-2025
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