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Osikapa bụ otu n'ime ihe ọkụkụ kachasị mkpa, nke sitere na ahịhịa ahịhịa mmiri nke ezinụlọ Poaceae. China bụ otu n'ime ebe obibi osikapa mbụ, nke a na-akọkarị na ndịda China na mpaghara ugwu ọwụwa anyanwụ. Site n'ọganihu nke sayensị na teknụzụ, a na-etinye usoro ihe ndị dị ndụ nke oge a na nyocha osikapa. Inweta ezigbo mma, DNA genomic osikapa dị elu na-atọkwa ntọala siri ike maka ọmụmụ mkpụrụ ndụ ihe nketa ala. Ngwa BigFish Magnetic Bead Based Rice Genomic DNA Purification Kit na-enyere ndị nyocha osikapa aka iwepụta DNA osikapa n'ụzọ dị mfe, ngwa ngwa na nke ọma.
Ngwa Rice Genome DNA dị ọcha
Nchịkọta ngwaahịa:
Ngwaahịa a na-eji sistemụ nchekwa pụrụ iche emepụtara yana kachasị mma yana bead ndọta nwere njirimara njide DNA akọwapụtara. Ọ na-ejikọta ngwa ngwa, na-ekesa ma na-ekewa nucleic acid ka ọ na-ewepụ ihe adịghị ọcha dị ka polysaccharides na ogige polyphenolic na osisi. Ọ dabara nke ọma maka iwepụta DNA genomic site na anụ ahụ akwụkwọ osisi. Ejikọtara ya na BigFish Magnetic Bead Nucleic Acid Extraction Instrument, ọ dị mma maka mmịpụta akpaaka nke nnukwu nlele. Ngwaahịa nucleic acid ewepụtara na-egosipụta ịdị ọcha dị elu na ịdị mma dị mma, na-eme ka ha bụrụ ndị ọdabara maka nyocha nnwale ala dị ka PCR/qPCR na NGS.
Njirimara ngwaahịa:
Nchekwa na nke anaghị egbu egbu: Ọ dịghị mkpa maka reagents organic na-egbu egbu dị ka phenol/chloroform
Nrụpụta dị elu akpaghị aka: Ejikọtara ya na Beagle sequencing nucleic acid extractor, ọ nwere ike ịrụpụta mmịpụta dị elu ma dabara adaba maka iwepụta oke nlele.
Ịdị ọcha dị elu na ezigbo mma: Ngwaahịa a amịpụtara nwere ịdị ọcha dị elu ma nwee ike iji ya mee ihe maka NGS ala, mgbawa mgbawa na nyocha ndị ọzọ.
Ngwa dakọtara: BigFish BFEX-32/BFEX-32E/BFEX-96E
Oge nzipu: Sep-11-2025
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