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N'ọhịa nkeNchọpụta ahụike n'ime vitro (IVD), genotyping, na nyocha molekul, ihe nlele ọnụ—dị kaswab ọnụ, swab akpịrị, na ọnụ mmiri- a na-ejikarị ha eme nnwale nucleic acid n'ihi na hanchịkọta dị mfe, ọdịdị na-adịghị emerụ ahụ, na usoro nlele ihe mgbu na-enweghị ihe mgbuAgbanyeghị, ihe nlele ọnụ na-enwekarịobere ọnụọgụ nke nucleic acidma na-emerụkarị ha naprotein na ihe ndị ọzọ na-adịghị mmaUsoro mwepụta ọdịnala na-enwekarị nsogbuusoro ọrụ dị mgbagwoju anya, obere arụmọrụ, na ojiji nke ihe ndị na-egbu egbu, nke nwere ike imebi izi ezi na nkwụsi ike nke ngwa ndị dị n'okpuru ala dịkaPCR/qPCR na usoro ọgbọ ọhụrụ (NGS).
IheNgwa Mwepụta DNA Genomic nke Dabere na Magnetic Bead nke BFMP06, nke mepụtara site naNkà na ụzụ ihe ndị dị na Hangzhou Bigfish, na-enye angwọta dị nchebe, dị irè, na nke a pụrụ ịtụkwasị obimaka mwepụta DNA nke ihe nlele ọnụ. Site na imewe teknụzụ ọhụrụ ya na ụkpụrụ arụmọrụ siri ike, ngwa a aghọọla ngwaọrụ a pụrụ ịtụkwasị obi maka ma ụlọ nyocha ahụike na nyocha.
E wuru ngwa BFMP06 gburugburusistemụ nchekwa kachasị mma pụrụ ichejikọtara ya naMkpụrụ osisi magnetik hydroxyl nke dị na DNA, na-emepụta usoro ọrụ nhicha nucleic acid dị irè nke ukwuu. Mgbe ewepụsịrị ihe nlele ahụ na lysis buffer, a na-emebi ihe ndị dị na sel ma wepụta nucleic acids. Otu ọrụ dị n'elu ihe magnetik ahụ na-ejikọta DNA efu, na-etolite kwụsiri ike.ihe mgbagwoju anya nke magnetic bead-DNA.
N'okpuru ebe ndọta mpụga, ihe mgbagwoju anya na-agafenzọụkwụ ịsacha abụọ kpọmkwemiwepụ protein, nnu, na ihe ndị ọzọ na-emerụ ahụ nke ọma. N'ikpeazụ,DNA genomic dị ọcha nke ukwuuA na-ewepụ ihe nchekwa elu nke ọma site na iji ihe nchekwa elu.
Nkọwa Ngwaahịa
Ngwaahịa a na-eji sistemụ nchekwa emebere nke ọma ma hazie ya yanaihe ndọta magnetik nke na-ejikọ DNA kpọmkwem, na-eme ka a na-amịnye, kewapụ, na nhicha nke nucleic acid ngwa ngwa. Ọ dabara nke ọma makamwepụ ngwa ngwa nke DNA genomic site na swabs ọnụ, swabs akpịrị, na ihe nlele mmiri, ebe ọ na-ewepụ protein na nnu ndị fọdụrụnụ nke ọma.
Mgbe ejiri ya na njikọta naNgwa mwepụta nke ihe e ji eme ka ihe dị n'ime mmiri nke Bigfish magnetik bead dịNgwa ahụ dị mma makamwepụta akpaaka dị eluDNA genomic e mere ka ọ dị ọcha bụ nkeịdị ọcha dị elu na mma dị mma, na-eme ka ọ dabara adaba maka ọtụtụ ngwa ndị dị n'okpuru ala, gụnyerePCR/qPCR na NGS.
Atụmatụ Ngwaahịa
Ịdị mma Dị elu
Na-ekewapụ ma na-asacha DNA genomic nke ọma site naswab ọnụ, swab akpịrị, na ọnụ mmiri, na-enyemmepụta dị elu na ịdị ọcha dị elu.
Ngwa ngwa ma dịkwa mfe
Achọghị usoro centrifugation ma ọ bụ nzacha vacuum ugboro ugboro. Dakọtara na ngwa mwepụ akpaka, nke na-eme ka ọ dị mma makanhazi ihe nlele buru ibu.
Nchekwa na Enweghị Nsí
Enweghị mkpa maka ihe ndị na-egbu egbu dịka ihe ndị dị ndụphenol ma ọ bụ chloroform.
Ngwa Ndị Dakọtara
Nnukwu azụ BFEX-16E
BFEX-32
BFEX-32E
BFEX-96
Nsonaazụ Nnwale
Ihe nlele swab ọnụ (mikpuru n'imeIhe ngwọta nchekwa 400 μL) na ihe nlele mmiri (Mmiri 200 μL + mmiri nchekwa 200 μL) e ji usoro nhazi ahụ hazie yaNgwa nhicha DNA nke BigfishE wepụrụ DNA naIhe nchekwa mmiri 70 μLma nyochaa site naElectrophoresis nke gel agarose, dịka egosiri na foto dị n'okpuru.
M: Ihe nrịbama DNA (2K Plus II)
Nkọwapụta Ngwaahịa
| Aha Ngwaahịa | Mba. Pusi. | Nkwakọ ngwaahịa |
| Ngwa nhicha DNA Genomic nke MagaPure Oral Swab (ngwugwu e tinyere tupu oge eruo) | BFMP06R | 32T |
| Ngwa nhicha DNA Genomic nke MagaPure Oral Swab (ngwugwu e tinyere tupu oge eruo) | BFMP06R1 | 40T |
| Ngwa nhicha DNA Genomic nke MagaPure Oral Swab (ngwugwu e tinyere tupu oge eruo) | BFMP06R96 | 96T |
| RNaseA (ịzụta) | BFRD017 | 1ml/pc (10mg/ml) |
Oge ozi: Jenụwarị-15-2026
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