Nnwale nke DNA jikọtara ọnụ na smartphones maka nyocha nke tumors na nyocha leukemia na izi ezi nke 90.0%!

Nchọpụta nke ọrịa cancer dabere na ndu mmiri nke ọrịa cancer na nchoputa nke US mba nke United States na-achọpụta ọrịa kansa ma ọ bụ ọbụna ọnya na-eme. E ji ya mee ihe n'ọtụtụ ebe dị ka akwụkwọ nyocha Biomarker maka mmalite nyocha nke ọrịa dị iche iche, gụnyere ọrịa akụrụ, eriri afọ, gliomas na ọrịa gynecouster.

Ihe ngosi nke ikpo okwu iji choputa methylation ala (Methylcapers nwere ike ime ka ihe di nma ka mma imeziwanye oria na - enwe oria na ogbo.

N'oge na-adịbeghị anya, ndị na-eme nchọpụta mepụtara ihe dị mfe ma na-eduzi ọdịdị ala methylation na-eme ka ya na ndị biostor doo (Cystenine chọpụtara na-eme nyocha ngwa ngwa nke ụdị tusers dịgasị iche iche. Enwere ike ịme nyocha nke nyocha na leukemia n'ime nkeji iri na ise ka ọpụpụ DNA site na nlele ọbara, yana izi ezi nke 90.0%. Isiokwu dị ngwa na-achọpụta ọrịa cancer DNA na ọbara mmadụ na-eji AUNSEMIENENENENENENENENENENENENENENENENENENENENENENT -ASESPL na smartphone na-enyere gị aka.

Ọgụgụ 1

Ihe a gosipụtara na eserese 1 A na-agbakwunye cyst / AUNPS na usoro agwakọta. DNA nkịtị na-emebi emebi nwere ihe dị iche iche dị iche iche, nke na-ebute iberibe DNA na usoro dị iche iche. Agụụ DNA na-agbaghakwa, emesịa chịkwaa cyst / AUNPS, nke na-ebute ọdịdị na-acha ọbara ọbara nke cyst / AUN nwere ike ịhụ ya na anya na-acha odo odo. N'ụzọ dị iche, profaịlụ metherụ pụrụ iche nke cancer DNA na-eduga na mmepụta nke ụyọkọ nke DNA.

Ejiri igwefoto na-enweghị akara ngosi 96. A na-atụle cancer DNA site na smartphone nwere igwe na-amụkwuru iji tụnyere usoro dị iche iche.

Inyocha cancer na ezigbo nlele

Exttentewanye ike nke ikpo okwu sinkụkọ, ndị ọrụ nyocha ahụ etinyere ihe mmetụta na-eme nke ọma n'etiti DNA na-arịa ọrịa kansa. Usoro methylation na saịtị CPG saịtị na-ekwupụta okwu okike. N'ihe fọrọ nke nta ka ọ bụrụ ụdị kansa niile, mgbanwe na DNA Methylition ma si otú a na mkpụrụ ndụ ihe nketa ndị na-akwalite tumugigesis.

Dị ka ihe nlereanya maka ọrịa cnaes ndị ọzọ, ndị ọrụ nyocha jiri ihe nlere nke ọrịa leukemia na njikwa ahụike nke methylape na ọrịa canceutimity leukan. Okirikiri methylape biomarker ọ bụghị naanị usoro nyocha nyocha Leukemia ngwa ngwa leukemia, kamakwa gosipụta na ọ ga-eme ka ị nweta oge nke ọrịa kansa dịgasị iche iche na nke kwụ ọtọ.

DNA sitere na ihe nlere sitere na ọbara site n'aka ndị ọrịa afọ iri na anọ na-ahụ maka ndị mmadụ iri na abụọ. Dịka e gosipụtara na igbe igbe 2a, onye ikwu gị na-anabata ihe nlele cancer (ΔA650 / 525) dị ala karịa nke DNA site na ụdị DNA. Nke a bụ n'ihi na nke a na-akwalite hydrophobity na-eduga na nchịkọta nke ọrịa cancer DNA, nke gbochiri nchịkọta nke cyst / AUNPS. N'ihi ya, nanoparticles ndị a chụpụrụ kpamkpam n'ígwé nke na-arịa ọrịa kansa, nke mere ka mgbasa nke cyst / AUNPS dị iche iche na canna na-arịa ọrịa. E mepụtara usoro nke roc site na ichebe ụzọ site na obere obere nke ΔA650 / 525 ruo uru kacha.

Ọgụgụ 2. (a) Uzo nke Akwukwo Nso nke Cyst / AUNPS Solutions na-egosi ọnụnọ nke nkịtị (na-acha anụnụ anụnụ) na kansa (uhie)

(Da650 / 525) nke igbe igbe; (b) nyocha ROC na nyocha nke ule nyocha. (c) Mintripy Matrix maka nchọpụta nke ndị ọrịa na-arịa kansa. (D) mmetụta uche, ịkọwapụta, ezigbo amụma bara uru (PPV), na-adịghị mma amụma uru (NPV) na izizi nke usoro emepe emepe.

Dị ka egosiri na eserese nke 2B, mpaghara dị n'okpuru roc usoro (Auc = 0.9274) nwetara maka ihe mmetụta na-egosi na nkọwapụta. Dị ka a pụrụ ịhụ site na igbe igbe ahụ, kachasi ike na-anọchi anya ndị otu DNA nkịtị site na isi kacha elu na-anọchi anya otu cancer cance; N'ihi ya, emere ka ọ bụrụ mgbanwe dị iche iche dị iche iche n'etiti òtù kansa na kansa. Nyere mgbanwe di iche iche nke di iche iche, ọ na - eme ka ihe omume nke ihe omume na - eme, dịka ọrịa kansa ma ọ bụ ìgwè nkịtị. Ntughari agbanwe agbanwe n'etiti 0 na 1. Nsonaazụ bụ ihe puru omume. Anyị kpebisiri ike na njirimara cancer (p) dabere na δA650 / 525 dị ka ndị a.

ebe b = 5.3533, w1 = -6.965. Maka nhazi ọkwa, ihe puru ime ihe na-erughị 0,5 na-egosi ezigbo nlele, ebe ihe puru omume nke 0,5 ma ọ bụ karịa na-egosi ihe nlele cancer. Ọgụgụ 2C na-egosi ọgba aghara ahụ site na ezumike - ọ na-abụkarị nke a na-eji eme ka nkwụsi ike nke usoro nhazi ọkwa. Ọgụgụ 2D na-achịkọta nyocha ule nyocha nke usoro ahụ, gụnyere mmetụta uche, ịkọwapụta uru dị mma (PPV) na uru amụma na-adịghị mma (NPV).

Mmalite sitere na Smaonurs

Iji mee ka ịnwale nlele dị mfe na-enweghị iji ụdị ụdị, ndị nyocha a na-eji ọgụgụ isi eme ihe (AI) ịkọwa agba nke ihe ngwọta na ịmata n'etiti ndị nkịtị na cancer. Nyere nke a, a na-eji ọhụụ kọmputa emegharị agba nke cyst / AUNPS ngwọta dị ka DNA (na-acha odo odo) site na iji ihe oyiyi 96 na-eji igwefoto ekwentị. Ọrụ ọgụgụ isi nwere ike belata ọnụego ma melite nnweta na ịkọwa agba nke ngwọta nke mgbịrịgba Nanoparticle. N'ikpeazụ, ụdị mmụta mmụta igwe abụọ, gụnyere oke ohia (rf) ma na-akwado igwe Vector (SVM) iji zụọ ụdị. Ma ụdị RF na SVM nke ọma na-ahazi ihe nlere ahụ dị ka nke ọma ma na-adịghị mma na izi ezi nke 90.0%. Nke a na-egosi na ojiji nke ọgụgụ isi na-eme ihe nkiri na ekwentị sitere na ekwentị-Ekwentị na-ekwe omume.

Ọgụgụ 3. (a) Classlọ ọrụ na-ahụ maka ihe ngwọta ahụ edekọtara n'oge nkwadebe nke ihe nlele maka nzọ nnweta onyonyo onyonyo. (b) Ihe atụ a na-eme ihe oyiyi n'oge nzọụkwụ ihe oyiyi. (c) ike agba nke cyst / aunps ngwọta na nke ọ bụla efere si na foto (b).

Iji cyst / AUNSP, ndị na-eme nchọpụta emepụtala nke ọma maka nchọpụta dị mfe na ihe mmetụta nwere ike ịmata ọdịiche dị na cancer DNA mgbe ị na-enyocha ihe nlele ọbara. Ihe mmetụta dị na DNA gosipụtara na DNA sitere na ezigbo nlele nwere ike ịchọpụta obere obere ọrịa cancer DNA na-arụ ọrụ nke ọma na nkeji iri na ise, ma gosipụta izi ezi nke 95.3%. Iji nweta ule nlele dị mfe site na iwepụ mkpa dị iche iche, a na-eji agụmakwụkwọ na-eme ka ọ dị iche iche na cancer.

Ihe: DOI: 10.1039 / D2RA05725e