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Ọganihu metụtara ya
Dị ka ụlọ ọrụ mgbasa ozi nke Ministry of Agriculture na ime obodo si kwuo, n'August 2018, ọrịa ezì Africa mere na Shenbei New District, Shenyang City, Liaoning Province, nke bụ ọrịa ezì mbụ nke Africa na China. N'ihe dị ka Jenụwarị 14, 2019, ọrịa ezì Africa emeela n'ihe karịrị mpaghara 20 na China, na-egbu ezì 916000, na-akpata nchegbu ọha.
Ahụ ọkụ ezì Africa (ASF)
ASF (African Swine fever) bụ ọrịa na-efe efe siri ike nke nje virus ezì nke Africa (ACFV) na-ebute ezì anụ ụlọ na anụ ọhịa (anụ ọhịa Africa, anụ ọhịa Europe, wdg). Òtù mba ụwa na-ahụ maka ahụike anụmanụ (OIE) depụtara ya dị ka ọrịa anụmanụ na-akọ n'ụzọ iwu kwadoro, nke bụkwa ụdị ọrịa anụmanụ na-efe efe nke China lekwasịrị anya n'igbochi.
Njirimara nke ọrịa ahụ
Ihe ngosi ụlọ ọgwụ bụ ahụ ọkụ (ruo 40 ~ 42 ℃), tachycardia, dyspnea, ụkwara akụkụ, serous ma ọ bụ mucinous purulent secretion na anya na imi, cyanosis akpụkpọ, ọbara ọgbụgba nke akụrụ, lymph nodes na eriri afọ mucosa. Ihe mgbaàmà ụlọ ọgwụ nke ahụ ọkụ ezì oge ochie nke Africa yiri nke ahụ ọkụ ezì oge gboo, nke enwere ike ịchọpụta ya naanị site na nlekota ụlọ nyocha.
Ngwọta maka ahụ ọkụ ezì oge gboo nke Africa
1. Nhazi nhazi
Ọ dabara maka ọbara na anụ ahụ dị iche iche nke ezì na-enyo enyo na-arịa ọrịa: splin, lymph node na akụrụ anụ ahụ.
Ihe nlele ọbara
Were ọbara 200 μ L, 5000g centrifugal 5 min, were supernatant maka nyocha.
Ihe nlele anụ ahụ
Mgbe ihe nlele anụ ahụ gbasachara nke ọma, a gbakwụnye saline ma ọ bụ PBS kwesịrị ekwesị, a na-etinyekwa ihe dị elu ka a nwalee ya.
2. Akpaaka mmịpụta
Hangzhou Bigfish Bio-tech Co., Ltd. BFEX-32 akpaka nucleic acid extractor nwere ike mezue mmịpụta nke 32 samples n'ime nkeji 30, nke nwere ike izute gị chọrọ nke wepụ a nnukwu ọnụ ọgụgụ nke samples. Usoro dum anaghị achọ ọrụ ọ bụla ọzọ, na-azọpụta oge ma na-ebelata njehie nke ọrụ ntuziaka, ma na-eme ka ọrụ ahụ dịkwuo mma dị ka o kwere mee.
3. Nnukwu ịdị ọcha nucleic acid dị ọcha
Ngwa mmịpụta acid MagPure Nucleic, yana BFEX-32, ngwaahịa ahụ dabara PCR na nchọpụta QPCR kpamkpam.
4. Nyocha na nyocha kọmputa
Dabere na ọnọdụ ụlọ nyocha ahụ n'ezie, onye ọrụ na-agbaso atụmatụ nchọpụta qualitative ma ọ bụ ọnụọgụ.
Hangzhou Bigfish Bio-tech Co., Ltd. na-enye ngwa nchọpụta qualitative (Quantitative) maka ọrịa ezì oge ochie nke Afrịka (ACFV) nwere FC-96G (BFQP-16/48), nke nwere ike ịchọpụta ACFV nke ukwuu, nke ọma na ntụkwasị obi.
Ọdịnaya ndị ọzọ, biko ṅaa ntị na akaụntụ gọọmentị WeChat nke Hangzhou Bigfish Bio-tech Co., Ltd.
Oge nzipu: Mee-23-2021
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