Ntuziaka maka ojiji nke Novel Coronavirus (SARS-CoV-2) Nnwale Ngwa Ngwa Antigen (Colloidal Gold)

【Okwu Mmalite】
Ọrịa coronavirus ọhụrụ a sitere na β genus. COVID-19 bụ ọrịa na-efe efe n'ikuku. Ndị mmadụ na-enwekarị ike ibute ya. Ugbu a, ndị ọrịa coronavirus ọhụrụ butere bụ isi ihe kpatara ọrịa ahụ; ndị nwere ihe mgbaàmà na-egosi na ha nwere ike ibute ọrịa nwekwara ike ịbụ ndị na-efe efe. Dabere na nyocha ọrịa na-efe efe ugbu a, oge mmalite ọrịa ahụ bụ ụbọchị 1 ruo 14, ọkachasị ụbọchị 3 ruo 7. Ihe ndị bụ isi na-egosi ya gụnyere ahụ ọkụ, ike ọgwụgwụ na ụkwara kpọrọ nkụ. A na-ahụ mkpọchi imi, imi mmiri, akpịrị mgbu, myalgia na afọ ọsịsa n'ọnọdụ ole na ole. Nchọpụta mbụ nke ndị butere ọrịa dị oke mkpa iji kwụsị mgbasa nke ọrịa a.
【Ojiji e zubere】
Nnwale Ngwa Ngwa Antigen Ọhụrụ (SARS-CoV-2) (Colloidal Gold) bụ ngwa nchọpụta njirimara in-vitro maka antigen nke coronavirus ọhụrụ nke a na-egosi na swabs Oropharyngeal mmadụ, swabs Anterior Imi, ma ọ bụ swabs Nasopharyngeal. Ngwa nnwale a bụ maka ndị ọkachamara ahụike na ụlọ nyocha naanị maka nchọpụta mbụ nke ndị ọrịa nwere mgbaàmà ahụike nke ọrịa SARS-COV-2.
Enwere ike iji ngwa nnwale a mee ihe n'ebe ọ bụla nke na-emezu ihe ntuziaka na iwu mpaghara chọrọ. Nnwale a na-enye naanị nsonaazụ nnwale mbụ. Nsonaazụ na-adịghị mma enweghị ike iwepụ ọrịa SARS-COV-2, a ga-ejikọkwa ha na nlele ahụike, akụkọ ihe mere eme na ozi gbasara ọrịa. Nsonaazụ nke ule a ekwesịghị ịbụ naanị ntọala maka nchọpụta ahụ; a chọrọ nnwale nkwenye.
【Usoro nnwale】
Ngwa nnwale a na-eji teknụzụ colloidal gold immunochromatography. Mgbe ngwọta mwepụta ihe nlele ahụ na-aga n'ihu n'akụkụ mpempe ule site na oghere ihe nlele ahụ gaa na pad na-amịkọrọ mmiri n'okpuru ọrụ capillary, ọ bụrụ na ngwọta mwepụta ihe nlele ahụ nwere antigen coronaviruses ọhụrụ, antigen ahụ ga-ejikọ na colloidal gold-labeled with anti-novel coronavirus monoclonal antibody, iji mepụta mgbagwoju anya ahụ ji alụso ọrịa ọgụ. Mgbe ahụ, a ga-ejide mgbagwoju anya ahụ ji alụso ọrịa ọgụ site na mgbochi ọzọ nke coronavirus monoclonal anti-novel, nke edobere na akpụkpọ ahụ nitrocellulose. Ahịrị agba ga-apụta na mpaghara nnwale "T", na-egosi antigen coronavirus ọhụrụ dị mma; Ọ bụrụ na ahịrị nnwale "T" egosighi agba, a ga-enweta nsonaazụ na-adịghị mma.
Kaseti nnwale ahụ nwekwara akara njikwa àgwà "C", nke ga-apụta n'agbanyeghị ma enwere ahịrị T a na-ahụ anya.
【Isi ihe mejupụtara】
1) Swab nje virus a na-atụfu nke a na-atụfu
2) Tube mwepụ nwere okpu nozzle na ihe nchekwa mwepụ
3) Kaseti nnwale
4) Ntuziaka maka Ojiji
5) Akpa ihe mkpofu na-emebi ihe
【Nchekwa na nkwụsi ike】
1. Chekwaa na okpomọkụ 4 ~ 30℃ nke na-anaghị apụta ìhè anyanwụ kpọmkwem, ọ dịkwa irè maka ọnwa 24 site na ụbọchị mmepụta.
2. Debe ya ka ọ kpọọ nkụ, ejikwala ngwaọrụ ndị kpọnwụrụ akpọnwụ na ndị nwụrụ anwụ.
3. A ga-eji kaseti ule mee ihe n'ime ọkara otu awa ozugbo emepere akpa foil Aluminom.
【Ịdọ Aka Ná Ntị na Ịkpachara Anya】
1. Ngwa a bụ maka nchọpụta in vitro naanị. Biko jiri ngwa ahụ n'ime oge nkwenye.
2. E bu n'obi ka a nwalee ule a iji chọpụta ọrịa COVID-19 dị ugbu a. Biko gakwuru ọkachamara ahụike ka ị kparịta maka nsonaazụ gị na ma ọ bụrụ na achọrọ nnwale ọzọ.
3. Biko debe ngwa ahụ dịka IFU si egosi, ma zere ọnọdụ oyi ogologo oge.
4. Gụọ ma soro ntuziaka ndị a nke ọma tupu i jiri ngwa ahụ, ma ọ bụghị ya, ihe na-ezighi ezi nwere ike ịnwe.
5.Ejila ihe ndị dị na ya dochie anya otu ngwa gaa na nke ọzọ.
6. Chebe onwe gị pụọ na mmiri mmiri, emeghela akpa platinum nke aluminum tupu ọ dị njikere maka nnwale. Ejila akpa foil nke aluminum mgbe a hụrụ na o meghere.
7. A ga-etinye ihe niile dị na ngwa a n'ime akpa mkpofu ihe na-akpata nsogbu nke ihe ndị dị ndụ ma tụfuo ya dịka iwu obodo si dị.
8. Zere ịwụfu ihe, ịgbasa ihe.
9. Debe ngwa nnwale na ihe ndị e ji eme ihe ebe ụmụaka na anụ ụlọ na-agaghị eru aka tupu na mgbe ejiri ya.
10. Jide n'aka na ìhè zuru oke mgbe ị na-anwale ya
11.Aṅụla ma ọ bụ tụfuo ihe nchekwa antigen n'akpụkpọ ahụ gị.
12. Ụmụaka na-erubeghị afọ 18 kwesịrị ịnwale ma ọ bụ duzie ha site n'aka onye toro eto.
13. Ọbara ma ọ bụ imi gabigara ókè n'ihe nlele swab nwere ike igbochi arụmọrụ ma nwee ike ịkpata nsonaazụ na-ezighi ezi.
【Nchịkọta na nkwadebe ihe atụ】
Nchịkọta ihe atụ:
Swab nke imi dị n'ihu
1. Tinye nsọtụ nchịkọta dum nke swab enyere n'ime oghere imi.
2. Jiri nwayọ tụọ ihe nlele ahụ n'ime mgbidi imi site n'ịtụgharị swab ahụ n'ụzọ okirikiri megide mgbidi imi ma ọ dịkarịa ala ugboro anọ.
3. Were ihe dị ka sekọnd iri na ise chịkọta ihe nlele ahụ. Jide n'aka na ị na-anakọta mmiri ọ bụla dị na imi nke nwere ike ịdị na swab ahụ.
4. Jiri otu swab ahụ megharịa ya n'oghere imi nke ọzọ.
5. Jiri nwayọ wepụ swab ahụ.
Nkwadebe ihe ngwọta ihe nlele:
1. Pụọ akpụkpọ ahụ mkpuchi ahụ n'ime tube mwepụta ahụ.
2. Tinye nsọtụ ákwà nke swab ahụ n'ime ihe nchekwa mwepụta dị na karama nke tube ahụ.
3. Gwakọta ma pịa isi swab ahụ n'elu mgbidi tube mwepụta ahụ iji wepụta antigen ahụ, na-atụgharị swab ahụ ruo otu nkeji.
4. Wepụ swab ahụ ka ị pịa ya na tube ahụ e ji ewepụta ihe.
(Hụ na ewepụrụ mmiri dị n'ọnụ ákwà ahụ dịka o kwere mee).
5. Pịa mkpuchi Nozzle nke e nyere nke ọma n'elu tube mwepụta ahụ iji zere mmiri ọ bụla nwere ike ịdapụta.
6. Tụfuo swab n'ime akpa ihe mkpofu nke ihe ize ndụ.