Ntuziaka dị mkpa na 8-Strip PCR Tubes: Na-agbanwegharị ọrụ ụlọ nyocha gị

N'ihe gbasara bayoloji molecular, izi ezi na arụmọrụ bụ ihe kacha mkpa. Otu ngwá ọrụ na-eme ka usoro nyocha ụlọ nyocha dịkwuo mma bụ 8-plex PCR tube. Emebere tubes ndị a ọhụrụ iji mee ka usoro mmeghachi omume polymerase (PCR) dị mfe, na-enye ndị nyocha ohere ịme nnwale n'ụzọ dị mfe na n'ụzọ ziri ezi. Na blọọgụ a, anyị ga-enyocha uru nke tubes PCR 8-plex, ndapụta ngwa, yana ndụmọdụ maka otu esi eme ka ikike ha dịkwuo elu na ụlọ nyocha.

Kedu ihe bụ 8-strip PCR tubes?

8-eriri PCR tubesna-mere nke asatọ iche iche PCR akpa ejikọrọ na usoro na-etolite a warara tube. Nke a imewe na-enye ohere ka otutu samples na-amụba n'out oge, na-eme ka ọ dị mma karịsịa maka elu-throughput nnwale. Igwe PCR ọ bụla dị na tube warara nwere ike ijide otu olu nke ngwakọta mmeghachi omume, na-abụkarị 0.1 ml ruo 0.2 ml, nke dabara maka ngwa PCR dị iche iche.

Uru nke iji 8-strip PCR tubes

1. Melite arụmọrụ: Otu n'ime uru kachasị dị ịrịba ama nke iji 8-strip PCR tubes bụ ịchekwa oge nkwadebe sample. Kama ijikwa tubes PCR n'otu n'otu, ndị nchọpụta nwere ike ibunye ọtụtụ ihe nlele n'otu oge, na-ebelata ihe ize ndụ nke mmetọ na njehie mmadụ.
2. Ọnụ ego na ịrụ ọrụ nke ọma: Site n'iji mpempe ule, ụlọ nyocha nwere ike ibelata ọnụ ọgụgụ ihe eji eme ihe achọrọ maka nnwale. Nke a abụghị naanị na-ebelata ụgwọ ọrụ, kamakwa ọ na-ebelata mmetụta nke plastik ndị a na-atụfu n'ike na gburugburu ebe obibi.
3. Nlebanya nlele emelitere: Ọtụtụ 8-strip PCR tubes na-abịa na n'ụzọ doro anya akara ebe, na-ekwe na-eme nnyocha na-adị mfe ịmata samples. Njirimara a dị mkpa na nnwale ebe nlele nlele ziri ezi dị oke mkpa iji hụ na nrụpụta nnwale.
4. Akpaaka dakọtara: Ka ụlọ nyocha na-enwetawanye teknụzụ akpaaka, imewe nke PCR tube 8-strip dịkwa dakọtara na sistemụ akpaaka. Ndakọrịta a na-abawanye ntinye ma na-akwado atụmatụ nnwale dị mgbagwoju anya.
5. Ntughari: Enwere ike iji tubes PCR 8-strip maka ngwa dị iche iche, gụnyere PCR quantitative (qPCR), PCR transcription (RT-PCR), na genotyping. Ntugharị ya na-eme ka ọ bụrụ ngwá ọrụ dị mkpa maka ọtụtụ ụlọ nyocha ihe ọmụmụ ihe omimi.

Ngwa nke 8-strip PCR tube

Ngwa nke tubes PCR 8-strip dị obosara ma dị iche iche. A na-ejikarị ha eme ihe maka:

• Nchọpụta ụlọ ọgwụ: N'ụlọ nyocha ahụike, enwere ike iji tubes PCR 8-strip iji chọpụta ọrịa na-efe efe ngwa ngwa, ọrịa mkpụrụ ndụ ihe nketa, na akara ọrịa kansa.
• Nnyocha na mmepe: N'ime ụlọ akwụkwọ nyocha na ụlọ ọrụ mmepụta ihe, tubes ndị a bara uru maka nchọpụta mkpụrụ ndụ ihe nketa, mmepe ọgwụ mgbochi, na ngwa ndị ọzọ dị ndụ.
• Sayensị forensic: Ikike ịkwalite DNA site na obere nlele na-eme ka tubes PCR 8-strip dị mkpa na nyocha nyocha, ebe ihe akaebe ọ bụla bara uru.

Ndụmọdụ maka ịbawanyewanye ojiji nke 8-strip PCR tubes

1. Welie ọnọdụ mmeghachi omume: Gbaa mbọ hụ na emebere ọnọdụ PCR maka nnwale gị akọwapụtara. Nke a na-agụnye ịhazigharị okpomọkụ na-akụda mmụọ, oge ndọtị, na ntinye enzyme.
2. Jiri reagents dị eluIhe ịga nke ọma nke PCR na-adabere n'ụzọ dị ukwuu n'ịdị mma nke reagents eji. Naanị site na ịhọrọ DNA polymerase, primers, na buffers nwere ike nweta nsonaazụ a pụrụ ịdabere na ya.
3. Nọgide na-amụ nwa: Iji gbochie mmetọ, na-eji usoro aseptic mgbe niile mgbe ị na-ejikwa tubes PCR 8-strip. Nke a na-agụnye iyi uwe aka, ịrụ ọrụ na gburugburu ebe dị ọcha, na izere mmetọ obe n'etiti ihe nlele.
4. Nchekwa kwesịrị ekwesị: Chekwaa tubes PCR 8-ejiri na-ejighi ya n'ebe dị jụụ, kpọrọ nkụ iji nọgide na-eguzosi ike n'ezi ihe. Soro ntuziaka nchekwa nke onye nrụpụta.

na ngwụcha

8-eriri PCR tubesbụ nkà na ụzụ na-akpaghasị ihe n'ihe gbasara ihe ndị dị ndụ, nwere ọtụtụ uru nwere ike imeziwanye arụmọrụ ụlọ nyocha na izi ezi. Site n'ịghọta uru na ngwa ya, ndị na-eme nchọpụta nwere ike iji ngwá ọrụ ndị a mee ka usoro ọrụ dịkwuo mma ma nweta nsonaazụ a pụrụ ịdabere na ya. Ma ị na-eme nyocha ụlọ ọgwụ, nyocha sayensị ma ọ bụ nyocha nyocha, itinye tubes PCR 8-strip n'ime omume ụlọ nyocha gị nwere ike melite arụmọrụ gị nke ọma. Nabata ọdịnihu nke PCR wee lelee nnwale gị ka ọ na-aga nke ọma na ngwọta ọhụrụ a!