Ngwa Nchọpụta Asịd Nucleic SARS-CoV-2 (Fluorescence RT-PCR)

Nkọwa Dị Mkpirikpi:

Enwere ike iji ngwa nchọpụta nucleic acid SARS-COV-2 (Fluorescence RT-PCR) maka nchọpụta nucleic acid nke coronavirus ọhụrụ, a na-eji ya maka nchọpụta enyemaka na nyocha epid-emiological nke ọrịa coronavirus ọhụrụ, nke dabara adaba maka CDC, ụlọ ọgwụ, ụlọ nyocha ahụike nke ndị ọzọ, ebe nyocha anụ ahụ na ụlọ nyocha ụlọ ọgwụ ndị ọzọ.

KPỌTỤRỤ ANYỊ

Nkọwa Ngwaahịa

Akara Ngwaahịa

Atụmatụ Ngwaahịa

1, Nnukwu mmetụta uche: Oke nchọpụta (LoD)＜Mbipụta 2 × 102/ml.

2, Mkpụrụ ndụ ihe nketa abụọ: Chọpụta mkpụrụ ndụ ihe nketa ORFlab na mkpụrụ ndụ ihe nketa N n'otu oge, soro iwu WHO.

3, Dabara adaba maka ngwaọrụ dị iche iche: ABI 7500/7500FAST; Roche LightCycler480; BioRad CFX96; BigFish-BFQP96/48 nke anyị.

4, Ngwa ngwa na mfe: Ngwakọta agwakọtara dị mfe iji, ndị ahịa chọrọ naanị itinye enzyme na template. Ngwa mwepụta nucleic acid nke Bigfish dabara nke ọma na nnwale a. Site na iji igwe mwepụ akpaka zuru oke, ọ dị ngwa ịhazi ọtụtụ ihe nlele..

5, Nchekwa Bio: Bigfish na-enye ihe nlele mmiri nchekwa iji gbochie nje virus ngwa ngwa iji hụ na nchekwa nke ndị ọrụ.