DNA/RNA mmịpụta

Iwebata ngwaahịa:

N'iji teknụzụ na-eme ka ọ dị ọcha magnetik, ngwa ihe nchacha nke DNA / RNA nwere ike wepụ DNA / RNA nke nje dị iche iche dị ka African Swine Fever virus na coronavirus ọhụrụ site na ụdị dị iche iche dị ka serum, plasma na swab immersion solution, ma nwee ike iji ya na PCR. /RT-PCR, sequencing, polymorphism analysis na ndị ọzọ nucleic acid nyocha na nchọpụta nchọpụta. Ejikọtara ya na NETRACTION ngwa nucleic acid na-eme ka ọ dị ọcha nke ọma na ngwa ebubata ngwa ngwa, nwere ike mezue mmịpụta nke ọnụ ọgụgụ buru ibu nke nucleic acid ngwa ngwa.

Njirimara ngwaahịa:

1. Dị mma iji, na-enweghị nsí reagent
2. Dị mfe iji, ọ dịghị mkpa maka Proteinase K na Carrier RNA
3. Wepụ nje virus DNA / RNA ngwa ngwa na nke ọma na oke uche
4. Ụgbọ njem na-echekwa na ụlọ okpomọkụ.
5. Kwesịrị ekwesị maka ịdị ọcha nke nucleic acid viral dị iche iche
6. Ejiri NUETRACTION ngwa ngwa nucleic acid dị ọcha zuru oke iji hazie 32 sample n'ime 30 mins.