Ngwa Mkpuchi DNA Genomic nke MagaPure Blood

Nkọwa Dị Mkpirikpi:

Ngwa a nwere obere microspheres superparamagnetic na ihe nchekwa mwepụta e mere tupu oge eruo, ọ dịkwa mma maka mwepụta DNA genomic dị mfe ma dị irè site na ihe nlele ọbara dum e ji aka kpuchie, nke e ji aka kpuchie, na nke e ji aka kpuchie ogologo oge. Mpekere DNA genomic ewepụtara buru ibu, dị ọcha nke ukwuu, ma dịkwa mma ma dịkwa mma. DNA ewepụtara dabara adaba maka nnwale dị iche iche dị n'okpuru ala dịka mgbaze enzyme, PCR, owuwu ụlọ akwụkwọ, njikọta Southern, na usoro mmepụta dị elu.

KPỌTỤRỤ ANYỊ

Nkọwa Ngwaahịa

Akara Ngwaahịa

Atụmatụ nke ngwaahịa

Ọtụtụ ngwa ihe atụ dị iche iche:A pụrụ iji mkpụrụ ndụ ihe nketa DNA wepụta ya ozugbo site na ihe nlele dịka ọbara mgbochi ikpụkọ ọbara (EDTA, heparin, wdg), akpụkpọ ahụ dị nro, na mkpụkọ ọbara.
Ngwa ngwa na mfe:A na-arụ ọrụ n'otu oge ijikọta ihe nlele na njikọ nucleic acid. Mgbe etinyere ihe nlele ahụ n'ime igwe ahụ, a na-emecha mwepụta nucleic acid na akpaghị aka, enwere ike nweta DNA genomic dị elu n'ime ihe karịrị nkeji iri abụọ.
Nchekwa na anaghị egbu egbu:Ihe na-eme ka ihe ahụ dị nro enweghị ihe mgbaze dị ka phenol na chloroform, o nwekwara ihe nchekwa dị elu.

Ngwaọrụ ndị a na-agbanwe agbanwe

Nnukwu azụ BFEX-32E/BFEX-32/BFEX-96E

Teknụzụ paramita

Ọnụọgụ ihe nlele:200μL
Mmetụta DNA:≧4μg
Ịdị ọcha nke DNA:A260/280≧1.75

Nkọwapụta

Aha Ngwaahịa	Mba. Pusi.	Nkwakọ ngwaahịa
Ngwa Mkpuchi DNA Genomic nke MagaPure Blood (Ngwugwu e tinyere tupu oge eruo)	BFMP02R	32T
Ngwa Mkpuchi DNA Genomic nke MagaPure Blood (Ngwugwu e tinyere tupu oge eruo)	BFMP02R1	40T
Ngwa Mkpuchi DNA Genomic nke MagaPure Blood (Ngwugwu e tinyere tupu oge eruo)	BFMP02R96	96T