Ngwa FFPE DNA dị ọcha

Nkọwa dị mkpirikpi:

Ngwa ahụ na-eji ngwọta dewaxing enyi na enyi na gburugburu ebe obibi wepụ DNA genomic dị elu site na ihe atụ nke paraffin agbakwunyere, na-emeri mmetụta mgbochi nke formalin cross-linking kpatara.
A na-agwakọta ihe nlele ahụ na ihe nchekwa mkpụrụ ndụ lysis iji gbajie mkpụrụ ndụ wee kpughee DNA. A na-agwakọta ihe nlele ahụ na ihe nchekwa mkpụrụ ndụ lysis iji mebie mkpụrụ ndụ ma kpughee DNA. A na-ejide DNA ahụ ekpughere site na ihe ndọta bead nanoparticles ka ọ bụrụ mgbagwoju anya-DNA. A na-ebufe ogige-DNA ndị ahụ na chaplet-DNA ogige ka ha na-asa ihe nchekwa iji wepụ oke adịghị ọcha. N'ikpeazụ, a na-ewepụ DNA ahụ ma nwetaghachi ya site na ibufe ya na nchekwa elution.