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Magapure FFPE genomic DNA dị ọcha ngwa
Okwu mmeghe dị nkenke
Ngwa a na-anabata usoro nchekwa pụrụ iche nke mepere emepe na nke kachasị mma yana beads magnetik nke na-ejikọ kpọmkwem na DNA, nke nwere ike jikọta ngwa ngwa, gbasaa, kewapụ ma sachaa acid nucleic. A na-eji usoro axing pụrụ iche iji gbawaa ma hapụ DNA n'ime akụkụ anụ ahụ, na-ebelata ihe ize ndụ nke imebi DNA kpatara site na ijikọta ụdọ molekụla nke anụ ahụ edoziri formalin kpatara. Site n'ịkwado iji Bigfish Magnetic Bead Nucleic Acid Extractor, ọ dabara nke ọma maka mmịpụta akpaaka nke nnukwu nlele. DNA genomic amịpụtara nwere ịdị ọcha na ịdị mma dị mma, enwere ike iji ya mee ihe na PCR / qPCR, NGS na nyocha nnwale ndị ọzọ.
Atụmatụ ngwaahịa
◆ Nchekwa na ntụkwasị obi: Ọ na-eji mmiri na-eme ihe na-adịghị mma na gburugburu ebe obibi, anaghị agụnye ihe mgbaze organic dị ka xylene, ọ dịghịkwa egbu egbu na adịghị emerụ ahụ.
◆ Ngwa ngwa ma dị mfe: A na-eji usoro bead magnet eme ihe maka mmịpụta na ịdị ọcha, yana nnukwu akpaaka na enweghị mkpa maka centrifugation multi-step.
◆Ọdịmma dị mma: DNA genomic amịpụtara nwere nnukwu itinye uche, ịdị ọcha na iguzosi ike n'ezi ihe, enwere ike iji ya mee nnwale ozugbo.
Ngwa nwere ike imeghari
Bigfish BFEX-32/BFEX-32E/BFEX-96E
Usoro nka
Ihe nlele: Mpekere 5-8 nke 5-10 μm
DNA dị ọcha: A260/280≧1.75
Nkọwapụta ngwaahịa
| Aha ngwaahịa | Nwamba. Mba. | Nkwakọ ngwaahịa |
| MagaDị ọchaFFPE GenomicNgwa DNA dị ọcha(pngwugwu ọzọ juru) | BFMP12R | 32T |
| MagaDị ọchaFFPE GenomicNgwa DNA dị ọcha (ngwungwu ejuruburu) | BFMP12R1 | 40T |
| MagaDị ọchaFFPE GenomicNgwa DNA dị ọcha (ngwungwu ejuruburu) | BFMP12R96 | 96T |
| RNase A(pichupu) | BFRD017 | 1 ml/tube (10 mg / ml) |
Ntọala nzuzo