Ngwa nhicha DNA genomic nke Magapure FFPE

Nkọwa Dị Mkpirikpi:

Ngwa a na-eji usoro nchekwa pụrụ iche emepụtara ma hazie ya na mkpụrụ osisi magnetik nke na-ejikọ kpọmkwem na DNA, nke nwere ike ijikọ ngwa ngwa, na-amịkọrọ, kewapụ ma sachaa asịd nucleic. A na-eji usoro pụrụ iche nke ịchapu igirigi iji gbawaa ma wepụta DNA na akụkụ anụ ahụ, na-ebelata ihe ize ndụ nke mmebi DNA nke sitere na njikọ nke agbụ molekul nke anụ ahụ formalin-fixed kpatara. Site n'ịkwado ojiji nke Bigfish Magnetic Bead Nucleic Acid Extractor, ọ dabara nke ọma maka mwepụta akpaaka nke nnukwu nha sample. DNA genomic ewepụtara nwere ịdị ọcha dị elu na ezigbo mma, enwere ike iji ya mee ihe nke ukwuu na PCR/qPCR, NGS na nyocha nnwale ndị ọzọ.

KPỌTỤRỤ ANYỊ

Nkọwa Ngwaahịa

Akara Ngwaahịa

Okwu mmeghe dị mkpirikpi

Atụmatụ nke ngwaahịa

◆Ọ dị mma ma dịkwa mma: Ọ na-eji mmiri dewaxing nke na-anaghị emebi gburugburu ebe obibi, ọ naghị agụnye ihe mgbaze organic dịka xylene, ọ naghịkwa egbu egbu ma ọ bụ na-emerụ ahụ.
◆Ọsọ na mfe: A na-eji usoro magnetik bead eme ihe maka mwepụ na ịsacha, yana oke akpaghị aka na enweghị mkpa maka centrifugation ọtụtụ nzọụkwụ.
◆Ọkwa dị mma: DNA genomic ewepụtara nwere nnukwu njupụta, ịdị ọcha na iguzosi ike n'ezi ihe, a pụkwara iji ya ozugbo maka nnwale ndị dị n'okpuru.

Ngwa ọrụ enwere ike ịgbanwe agbanwe

Nnukwu azụ BFEX-32/BFEX-32E/BFEX-96E

Teknụzụ paramita

Olu ihe atụ: Mpekere 5-8 nke 5-10 μm
Ịdị ọcha nke DNA: A260/280≧1.75

Nkọwapụta nke ngwaahịa

Aha Ngwaahịa	Pusi. Mba.	Nkwakọ ngwaahịa
MagaDị ọchaFFPE GenomicNgwa Nchacha DNA(pngwugwu e mejugharịrị)	BFMP12R	32T
MagaDị ọchaFFPE GenomicNgwa Nchacha DNA (ngwugwu e tinyere tupu oge eruo)	BFMP12R1	40T
MagaDị ọchaFFPE GenomicNgwa Nchacha DNA (ngwugwu e tinyere tupu oge eruo)	BFMP12R96	96T
RNase A(purchase)	BFRD017	1ml/tube (10mg/ml)