Ngwa dị ọcha nke DNA Genomic MagaPure

Ngwa dị ọcha nke MagaPure Blood Genomic DNA egosipụtara

Nkọwa dị mkpirikpi:

Ngwa a nwere microspheres superparamagnetic na ihe nchekwa mmịpụta emebere emebere, ọ dabara adaba maka mmịpụta DNA genomic dị mfe na nke ọma site na nlele ọbara zuru oke, oyi kpọnwụrụ na ogologo oge echekwara. Iberibe DNA genomic ndị ahụ ewepụtara buru ibu, dị oke ọcha, ma nwee ogo kwụsiri ike na ntụkwasị obi. DNA ewepụtara dabara adaba maka nnwale dị iche iche dị n'okpuru mmiri dị ka mgbaze enzyme, PCR, iwu ụlọ ọba akwụkwọ, ngwakọ ndịda, na usoro nrụpụta dị elu.

KPỌTỤRỤ ANYỊ

Nkọwa ngwaahịa

Mkpado ngwaahịa

Atụmatụ ngwaahịa

Ngwa nlele obosara:Enwere ike ị nweta DNA genomic ozugbo site na nlele dị ka ọbara anticoagulated (EDTA, heparin, wdg), uwe mwụda, na mkpụkọ ọbara.
Ọsọ na mfe:A na-eme lysis sample na njikọ nucleic acid n'otu oge. Mgbe etinyere ihe nlele ahụ na igwe, a na-emecha mmịpụta nucleic acid na-akpaghị aka, enwere ike nweta DNA genomic dị elu n'ime ihe karịrị nkeji iri abụọ.
Nchekwa na anaghị egbu egbu:Reagent enweghị ihe mgbaze na-egbu egbu dị ka phenol na chloroform, ma nwee ihe nchekwa dị elu.

Ngwa emeghari

Bigfish BFEX-32E/BFEX-32/BFEX-96E

Usoro nka

Ọnụego nlele:200μL
Ntinye DNA:≧4μg
DNA dị ọcha:A260/280≧1.75

Nkọwapụta

Aha ngwaahịa	Nwamba. Mba.	Nkwakọ ngwaahịa
Ngwa MagaPure Blood Genomic DNA Purifification Kit (ngwungwu ejuburu)	BFMP02R	32T
Ngwa MagaPure Blood Genomic DNA Purifification Kit (ngwungwu ejuburu)	BFMP02R1	40T
Ngwa MagaPure Blood Genomic DNA Purifification Kit (ngwungwu ejuburu)	BFMP02R96	96T