Ngwa ihe nchacha DNA nke nje MagPure

Nkọwa Dị Mkpirikpi:

Mgbe ejiri mmiri nri gwọọ ya, a na-ewepụta nucleic acid dị na ihe nlele nje bacteria naanị site na iji Lysis Buffer. DNA genomic ewepụtara na-ejikọta naanị na Magnetic beads. A na-ejide DNA genomic nke ejikọtara na ihe magnetik site na ihe magnet; a na-ewepụ ihe ndị na-emerụ ahụ site na ịsa ahụ na Wash Buffer. A na-ewepụ nucleic acid site na ihe ndị ahụ site na iji Elution Buffer. Ọ dị irè maka ụdị nje bacteria niile na-adịghị mma na nje bacteria dị mma, wdg.

KPỌTỤRỤ ANYỊ

Nkọwa Ngwaahịa

Akara Ngwaahịa

Isi ihe mejupụtara ya

Pusi.#	BFMP10R1	BFMP10R16	BFMP10R	Cihe ndị dị mkpa
Packing	1T40T/ngwa	16T16T/ngwaọrụ	32T32T/ngwaọrụ
Protease K	400μ L x 2	320μL	640μ L	Ngwọta protease (E ji ya iche iche)
RNaseA	80μL	32μL	64μ L	Ngwọta enzyme (E ji ya rụọ ọrụ iche)
Buffer BA	12ml	5ml	10ml	Ngwọta nnu
Buffer BL	N'ime olulu mmiri iri anọ na isii nkwadebe. katrij Mpekere iri anọ.	96 nke ọma eburula ụzọ jupụta efere 2 pcs.	96 nke ọma eburula ụzọ jupụta efere 2 pcs.	Denaturant siri ike na ihe nchekwa Tris
Buffer WA				Ngwọta nnu
Nchekwa WB				Ngwakọta saline dị obere
Nchekwa DE				Ngwọta Tris
Magnetik beads				Ngwọta hydroxy magnetik
Akwụkwọ ntuziaka ndị ọrụ	1	1	1