Igwe nyocha Ultramikroskoprofotometer

Okwu Mmalite Ngwaahịa

Ultramicrospectrophotometer bụ ụdị nchọpụta ngwa ngwa na nke ziri ezi nke njupụta nucleic acid, protein na sel na-enweghị ọkụ tupu oge eruo, nha ihe nlele naanị 0.5 ruo 2ul, ụdị cuvette nwekwara ike ịchọpụta mkpokọta nke nje bacteria na ihe ndị ọzọ metụtara ọdịbendị. Enwere ike ijikọ ọrụ nchọpụta fluorescence na ngwa nyocha fluorescence quantitative, site na njikọta kpọmkwem nke agba fluorescence na ihe ndị a na-elekwasị anya nwere ike ịtụle nke ọma ọnụọgụ DNA, RNA na protein, nke kacha nta nwere ike iru 0.5pg/μl (dsDNA).

Atụmatụ ngwaahịa

Ugboro ọkụ ọkụ dị mkpụmkpụ, ma e jiri ya tụnyere ụzọ nchọpụta ọdịnala iji mee ka ndụ ọrụ nke isi iyi ọkụ dịkwuo elu. Mkpali ọkụ nke obere ngwaahịa nnwale nwere ike ịbụ nchọpụta ngwa ngwa, ọ gaghị adị mfe imebi.;

Ọrụ Fluorescence: Site na fluorescence quantitative reagent, enwere ike ịchọpụta mkpokọta pg dsDNA.;

Teknụzụ nchọpụta ụzọ anya 4: teknụzụ njikwa moto pụrụ iche, ojiji nke ụdị nchọpụta ụzọ anya "4", nkwụsi ike, imeghachi omume, linearity ka mma, oke nha buru ibu;

Igwe nbipute arụnyere n'ime ya: Site na nhọrọ dị mfe iji data-na-ebi akwụkwọ, ị nwere ike ibipụta akụkọ ozugbo site na mbipụta arụnyere n'ime ya.r;

Ngwọta nje bacteria OD600, nchọpụta nje bacteria: na sistemụ nchọpụta ụzọ anya OD600, karịa ụdị nri dị mma maka nje bacteria, obere nje na nchọpụta njupụta nke ihe ngwọta ọdịbendị ndị ọzọ;

Nnukwu imeghachi ihe na linearity;